Recombinant adenovirus-mediated expression of GHS-R1a in HEK 293 cells
نویسندگان
چکیده
منابع مشابه
High-yield production of recombinant antibody fragments in HEK-293 cells using sodium butyrate.
To develop new recombinant monoclonal antibody fragments for therapy and imaging, it is indispensable to have a simple and easy procedure to handle the eukaryotic expression system for production of proteins in high amounts. Gene amplification techniques such as the dehydrofolate reductase (DHFR) system in Chinese hamster ovary cells or the glutamine synthase system in myeloma cells have a coup...
متن کاملInfluence of glutamine on transient and stable recombinant protein production in CHO and HEK-293 cells
Background Glutamine is an essential component in culture media for most of the mammalian cell lines. It is often used as an alternative source of energy by cells, along with glucose. Glutamine metabolism induces ammonia accumulation in cell culture. Elevated ammonia concentration above 2 mM has been shown to have negative impact on both cell growth and recombinant protein productivity [1-4]. I...
متن کاملThe ethics of HEK 293.
1 Congregation for the Doctrine of the Faith, Donum vitae (February 2, 1987), Introduction, 5. 2 Ibid., I, 1. The topic of embryonic stem cells is very much in the forefront of public interest. The moral stance on obtaining stem cells from embryos is clear: The Church has always taught that all human life from the very beginning must be respected and protected. If human embryos are killed to ob...
متن کاملCharacterization of a Mutant Dopamine Transporter in HEK-293 Cells
................................................. 3 Background.............................................. 4 Materials and Methods...................................8 Results....................................................12 Discussion................................................14 Acknowledgments.......................................16 References.........................................
متن کاملGrowth hormone-releasing hormone as an agonist of the ghrelin receptor GHS-R1a.
Ghrelin synergizes with growth hormone-releasing hormone (GHRH) to potentiate growth hormone (GH) response through a mechanism not yet fully characterized. This study was conducted to analyze the role of GHRH as a potential ligand of the ghrelin receptor, GHS-R1a. The results show that hGHRH(1-29)NH(2) (GHRH) induces a dose-dependent calcium mobilization in HEK 293 cells stably transfected with...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Neuroscience Bulletin
سال: 2010
ISSN: 1673-7067,1995-8218
DOI: 10.1007/s12264-010-1228-1